Document 3226
 DOCN  M94A3226
 TI    Primary infection in lymph nodes of macaques inoculated with pathogenic
       and attenuated SIV.
 DT    9412
 AU    Chakrabarti L; Cumont MC; Baptiste V; Khatissian E; Montagnier L;
       Hurtrel B; Unite d'Oncologie Virale, Institut Pasteur, Paris, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):124 (abstract no. PA0116). Unique
       Identifier : AIDSLINE ICA10/94369349
 AB    Our purpose was to characterize the very early events that distinguish
       pathogenic and attenuated SIVmac infections. Using in situ
       hybridization, we determined the kinetics of viral spread in lymph nodes
       (LN) obtained sequentially during the primary stage of infection. 4
       Rhesus macaques were inoculated intravenously with a high dose of the
       pathogenic isolate SIVmac 251. 4 macaques received a low dose (10 AID50)
       of the same isolate. 3 macaques received a high dose of an attenuated
       virus derived from the SIVmac 251 BK28 clone with a deletion in the nef
       gene (251 delta nef). 3 macaques received a high dose of the virus
       derived from the SIVmac 239 clone with a stop codon in the nef gene (239
       nef stop). For each animal, 4 axillary or inguinal LN were taken
       sequentially between 7 days and 2 month post-inoculation (p.i.). Primary
       LN infection with the pathogenic SIVmac 251 consisted in 2 phases, with
       a massive viral replication in lymphocytes and macrophages, followed by
       an accumulation of viral particles in the germinal centers. The same
       phases were observed with attenuated SIV, though the number of
       productively infected cells was reduced by a factor 2 to 10. The 239 nef
       stop virus persisted at higher levels in LN than the 251 delta nef
       virus, probably because of a rapid reversion of the stop codon in the
       nef gene. Interestingly, germinal center differentiation occurred
       earlier in animals infected with attenuated viruses, pointing to
       differences in the development of the humoral response.
 DE    Animal  Comparative Study  Gene Deletion  Genes, nef  In Situ
       Hybridization  Lymph Nodes/*MICROBIOLOGY  Lymphocytes/MICROBIOLOGY
       Macaca mulatta  Macrophages/MICROBIOLOGY  Simian Acquired
       Immunodeficiency Syndrome/MICROBIOLOGY/*PATHOLOGY  SIV/*ISOLATION &
       PURIF/PHYSIOLOGY/PATHOGENICITY  Virus Replication  MEETING ABSTRACT

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